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#9869 Cyclin Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#9869T1 Kit107,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Cyclin A2 (BF683) Mouse mAb #4656 20 µl WB H 55 Mouse IgE
Cyclin B1 Antibody #4138 20 µl WB, IF-IC H, M, R, Hm, Mk 55 Rabbit
Cyclin D1 (92G2) Rabbit mAb #2978 20 µl WB, IHC-P, F H, M, R 36 Rabbit IgG
Cyclin D2 (D52F9) Rabbit mAb #3741 20 µl WB, IP H, M, R 31 Rabbit IgG
Cyclin D3 (DCS22) Mouse mAb #2936 20 µl WB, IHC-P H, M, R 31 Mouse IgG1
Cyclin E1 (HE12) Mouse mAb #4129 20 µl WB H, Mk 48 Mouse IgG1
Cyclin E2 Antibody #4132 20 µl WB H C 48 Rabbit
Cyclin H Antibody #2927 20 µl WB, IP H, M, R 36 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl WB Horse

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey

貯法
-20℃
社内データ

Western blot analysis of extracts from various cell lines using Cyclin D2 (D52F9) Rabbit mAb #3741.

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC, C6 and IMCD3 cells, using Cyclin D3 (DCS22) Mouse mAb.

Western Blotting

Western Blotting

Western analysis of extracts from Hela cells that were untreated, treated with doxorubicin (0.5 µM, 24 hours), or with nocodazole (50 ng/ml, 24 hours), using Cyclin A2 (BF683) Mouse mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and Raji cells, using Cyclin H Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7, SK-N-MC and HeLa cells, untreated or treated with the proteasome inhibitor MG-132, using Cyclin E2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7, L929 and C6 cells, using Cyclin D1 (92G2) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Cyclin E (HE12) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Cyclin B1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Cyclin D2 (D52F9) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin D3 (DCS22) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cyclin D1 (92G2) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Cyclin B1 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red) showing concentrated cyclin B1 staining surrounding mitotic chromatin.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cyclin D3 (DCS22) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Apc (min/+) mouse intestine using Cyclin D1 (92G2) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin D1 (92G2) Rabbit mAb in the presence of control peptide (left) or Cyclin D1 Blocking Peptide #1044 (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded H1975 xenograft, using Cyclin D1 (92G2) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated HT29 cells, using Cyclin D1 (92G2) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

バックグラウンド

Control of the cell cycle is regulated by a multitude of cellular events and processes. The cyclin dependent kinases (CDK) regulate many of these pathways and constitute an active complex when associated with their cyclin partners. This activity is controlled primarily by phosphorylation, which determines subcellular localization of the CDK/cyclin complex (1,2). Some phosphorylation events control the function of cytoplasmic retention sequences while other events regulate nuclear localization and export sequence function (3,4). Cyclin and cyclin-dependent kinase inhibitor (CKI) levels are regulated by ubiquitination and degradation via the ubiquitin proteasome pathway (5). A variety of CKI proteins associate with these complexes and modulate access to regulatory domains on cyclins (6). Additional complexity is generated as the controlled protein levels of each cyclin oscillate with the stages of cell cycle. Increased expression of Cyclin D1 is associated with certain types of cancer (7,8) and may associate with TSC2 (tuberin) independent of its Cdk partner (9).

使用文献
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9868   CDK Antibody Sampler Kit

Tween is a registered trademark of ICI Americas, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Cyclin Antibody Sampler Kit

Metabolic Reprogramming in Disease

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