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#98785 OX40 (ACT35) Mouse mAb

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2019年1月15日15時15分 現在
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#98785S100 μL58,000
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TNFRSF4 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性35-50Mouse IgG2a-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト -
98785 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫組織染色 (パラフィン) (1:400)免疫蛍光細胞染色 (IF-IC) (1:1000)フローサイトメトリー (1:200)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
OX40 (ACT35) Mouse mAb recognizes endogenous levels of total OX40 protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with HuT 102 cell line.

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社内データ

※下記の社内データは、すべて98785 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from HuT 102, human CD4+ T, and Jurkat cells using OX40 (ACT35) Mouse mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from blood, and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Interleukin-2 (hIL-2) #8907 (20 ng/ml).

IP

IP

Immunoprecipitation of OX40 protein from HuT 102 cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is OX40 (ACT35) Mouse mAb. Western blot analysis was performed using

OX40 (ACT35) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HuT 102 cell pellet (left, positive) or Jurkat cell pellet (right, negative) using OX40 (ACT35) Mouse mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using OX40 (ACT35) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using OX40 (ACT35) Mouse mAb performed on the Leica® BOND™ Rx.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using OX40 (ACT35) Mouse mAb performed on the Leica® BOND™ Rx.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using OX40 (ACT35) Mouse mAb (left) compared to concentration matched Mouse (G3A1) IgG1 Isotype Control #5415 (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HuT 102 cells (left, positive) and Jurkat cells (right, negative) using OX40 (ACT35) Mouse mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells (blue) and HuY 102 cells (green) using OX40 (ACT35) Mouse mAb (solid lines) or a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.


バックグラウンド

OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12).

使用例
 
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14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
6883   SignalFire™ ECL Reagent
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
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8125   SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
LEICA is a registered trade​mark of Leica Microsystems IR GmbH.
ProLong is a registered trademark of Life Technologies Corporation.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

OX40 (ACT35) Mouse mAb

Immune Cell Signaling Pathways

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