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#9901 LY294002

  • LY294002 Data Sheet PDF

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CSTコード 包装
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2019年3月22日15時35分 現在
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#9901S1.5 mg13,000
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概要
PI3 Kinase を選択的に抑制します。PI3 Kinase 依存のAkt タンパク質のリン酸化やキナーゼ活性も阻害します。
50 % 阻害濃度
1.4 μM
分子量 
307.35
分子の化学式
C19H17NO3
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from PDGF, wortmannin and LY294002 treated NIH/3T3 cells, using Phospho-Akt (Ser473) Antibody #9271 (A), Phospho-Akt (Thr308) Antibody #9275 (B) or Akt Antibody #9272 (C). D is an analysis of Akt activity in PDGF, wortmannin and LY294002 treated NIH/3T3 cells, using Akt Kinase Assay Kit #9840.

Structure

Structure

Molecular Formula: C19H17NO3

バックグラウンド

LY294002 was shown to act in vivo as a highly selective inhibitor of phosphatidylinositol 3 (PI3) kinase. When used at a concentration of 50 μM, it specifically abolished PI3 kinase activity (IC50=0.43 μg/ml; 1.40 μM) but did not inhibit other lipid and protein kinases such as PI4 kinase, PKC, MAP kinase or c-Src (1). LY294002 is soluble in DMSO or ethanol. For use with in vitro or cell-based assays, it may be diluted into aqueous buffers to yield the desired concentrations. For experiments with cultured cells, CST recommends treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation. LY294002 has been shown to block PI3 kinase-dependent Akt phosphorylation and kinase activity.

使用文献
 
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9271   Phospho-Akt (Ser473) Antibody
9275   Phospho-Akt (Thr308) Antibody
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

LY294002

Immune Cell Signaling Pathways

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