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#9920 Phospho-(Ser/Thr) Kinase Substrate Antibody Sampler Kit

 
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2019年1月18日15時10分 現在
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#9920T1 Kit92,000
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Kinase substrate 製品一覧

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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
Phospho-AMPK Substrate Motif [LXRXX(pS/pT) MultiMab™ Rabbit mAb mix #5759 20 µl WB, IP, E-P All Rabbit
Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614 20 µl WB, IP, IHC-P, E-P H, M, Dm, All Rabbit IgG
Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb #9624 20 µl WB, IP, E-P All Rabbit IgG
Phospho-ATM/ATR Substrate Motif [(pS/pT) QG] MultiMab™ Rabbit mAb mix #6966 20 µl WB, IP All Rabbit IgG
Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab™ Rabbit mAb mix #6967 20 µl WB, IP, E-P All Rabbit IgG
Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix #9477 20 µl WB, IP, E-P H, All Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA), IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: All=All Species Expected, H=Human, M=Mouse, Dm=D. melanogaster

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved NIH/3T3 cells, phosphorylated in vitro with Akt kinase, or treated in culture with PDGF or FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved A431 cells, phosphorylated in vitro with PKA kinase or treated in culture with forskolin/IBMX, using Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb. Lysis buffer: 1.0% Triton X-100 (lanes 1 and 2), 2.0% SDS (lanes 3 and 4).


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or UV-treated (+, 50 mJ/cm2, 2 hr), using

Phospho-ATM/ATR Substrate Motif [(pS/pT) QG] MultiMab™ Rabbit mAb mix. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biotechnologies).

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or TPA-treated (200 nM, 30 min; +), using Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab™ Rabbit mAb mix. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biotechnologies).

Western Blotting

Western Blotting

Western blot analysis of extracts from various mouse tissues using Phospho-AMPK Substrate Motif [LXRXX(pS/pT) MultiMab™ Rabbit mAb mix


Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, synchronized in G1/S, G2/M, and G0 phase of the cell cycle, using

Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix.

IP

IP

Immunoprecipitation of extracts from serum-starved NIH/3T3 cells, untreated or treated with FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb, followed by Western blot analysis using the same antibody.

IP

IP

Immunoprecipitation of Hela cells, untreated (-) or UV-treated (+, 50 mJ/cm2, 2 hr), using

Phospho-ATM/ATR Substrate Motif [(pS/pT) QG] MultiMab™ Rabbit mAb mix (lanes 3 and 4). Lanes 1 and 2 show 10% input. Western blot detection was performed using the same antibody (upper) and p95/NBS1 Antibody #3002 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from various mouse tissues using Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab™ Rabbit mAb mix.

Western Blotting

Western Blotting

Western blot analysis of extracts from H1650 cells, untreated or treated with phenformin (5 mM, 1 hr), using

Phospho-AMPK Substrate Motif [LXRXX(pS/pT) MultiMab™ Rabbit mAb mix. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biosciences).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.


IP

IP

Immunoprecipitation (IP) of extracts from Jurkat cells, untreated (-) or TPA-treated (200 nM, 30 min; +) (lanes 3 and 4), using Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab™ Rabbit mAb mix. 10% input is shown in lanes 1 and 2. Western blot analysis was performed using the same antibody.

IP

IP

Immunoprecipitation of extracts from H1650 cells using Phospho-AMPK Substrate Motif [LXRXX(pS/pT) MultiMab™ Rabbit mAb mix. Lane 1 shows 10% input. Western blot analysis was performed using the same antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded renal cell carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.

バックグラウンド

Phospho-(Ser/Thr) kinases and phosphatases play critical roles in a wide range of biological processes. Each phospho-(Ser/Thr) kinase phosphorylates serine or threonine within a specific motif. Akt phosphorylates substrates at a serine or threonine only in a conserved motif characterized by arginine at positions -5 and -3 (1). Conventional PKC isozymes phosphorylate substrates containing serine or threonine, with arginine or lysine at the -3, -2 and +2 positions, and a hydrophobic amino acid at position +1 (2,3). A consensus phosphorylation site of PKA is serine or threonine with arginine at the -2 and -3 positions (3). AMPK phosphorylates consensus motif (L/M)XRXX(S/T)XXXL (6). Antibodies recognizing the LXRXX(S/T) motif are very useful in the identification of AMPK substrates. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where denotes any one of the 20 amino acids and S* is the phosphorylation site (4-6). ATM and the related kinase ATR phosphorylate serine or threonine in an S*/T*Q motif (7,8).

Antibodies specific to particular kinase substrates are invaluable reagents in determining kinase activity and identifying potential new kinase substrates. CST has developed antibodies that recognize phosphorylated serine or threonine within the context of a protein motif that is phosphorylated by Akt, PKC, PKA, MAPK/CDK, CDKs or ATM/ATR. As shown by peptide pairing ELISA, each phospho-(Ser/Thr) kinase substrate antibody in this sampler kit is specific to its kinase substrate motif.

使用文献
 
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2351   Phospho-Threonine-X-Arginine Antibody
2909   Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb
2981   Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9601   Phospho-(Ser) 14-3-3 Binding Motif Antibody
9606   Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb
9631   Phospho-(Ser/Thr) Phe Antibody
9634   Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.
LI-COR is a registered trademark of LI-COR, Inc.
Odyssey is a registered trademark of LI-COR, Inc.
MultiMab is a trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

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