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#9928 Histone Deacetylase (HDAC) Antibody Sampler Kit

 
CSTコード 包装
希望納入価格 (円)
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2019年1月17日15時10分 現在
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#9928T1 Kit92,000
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HDAC 製品一覧

9928 の推奨プロトコール i

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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
HDAC1 (10E2) Mouse mAb #5356 20 µl WB, IP H, M, R, Mk 62 Mouse IgG1
HDAC2 (3F3) Mouse mAb #5113 20 µl WB, IP, IF-IC H, M, R, Mk 60 Mouse IgG1
HDAC3 (7G6C5) Mouse mAb #3949 20 µl WB, IP, IF-IC H, M, R, Mk 49 Mouse IgG2a
HDAC4 (D15C3) Rabbit mAb #7628 20 µl WB, IP H, M, R, Mk 140 Rabbit IgG
Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443 20 µl WB H, M 140, 124 Rabbit IgG
HDAC6 (D2E5) Rabbit mAb #7558 20 µl WB, IP, IHC-P, IF-IC, F H, Mk 160 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl WB Horse

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC3 (7G6C5) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC4 (D15C3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of NIH/3T3 cells using Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5)

Rabbit mAb #3443, HDAC4 (D8T3Q) Rabbit mAb #15164, HDAC5 (D1J7V) Rabbit mAb #20458, and HDAC7 (D4E1L) Rabbit mAb #33418. To show the phospho-specificity of the antibody, the blot was mock treated (-) or treated (+) with calf intestinal phosphatase (CIP) and lambda phosphatase. As expected, signal from the Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb is lost after treatment of the blot with phosphatase.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using HDAC3 (7G6C5) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using HDAC2 (3F3) Mouse mAb (green). Actin filaments were labeled using DY-554 Phalloidin (red).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC6 (D2E5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HDAC6 (D2E5) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells using HDAC6 (D2E5) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with MG132 (5 μM, 24 hr; right), using HDAC6 (D2E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).

バックグラウンド

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

使用例
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DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Histone Deacetylase (HDAC) Antibody Sampler Kit

Immune Cell Signaling Pathways

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