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#9945 Jak Isoform Sampler Kit

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希望納入価格 (円)
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2019年1月18日15時10分 現在
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#9945T1 Kit61,000
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Jak 製品一覧

9945 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Jak1 (6G4) Rabbit mAb #3344 20 µl WB, IHC-P H, M, R Mk, Dg, Pg 130 Rabbit IgG
Jak2 (D2E12) XP® Rabbit mAb #3230 20 µl WB, IP, IHC-P, IF-IC, F H, M, R Hm, Mk, C, X, B, Dg, Pg, Hr 125 Rabbit IgG
Jak3 (D1H3) Rabbit mAb #8827 20 µl WB, IP H Mk 115 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat

貯法
-20℃
※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from ACHN, SR, CTLL-2 and PC12 cell lines using Jak1 (6G4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or Jak2 siRNA (+), using Jak2 (D2E12) XP® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Jak2 siRNA.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Jak3 (D1H3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb #3344, in the presence of control peptide (left) or Jak1 Blocking Peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak1 (6G4) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mA in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).


バックグラウンド

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
1039   Jak2 Blocking Peptide
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
3331   Phospho-Jak1 (Tyr1034/1035) Antibody
3771   Phospho-Jak2 (Tyr1007/1008) Antibody
3774   Phospho-Jak2 (Tyr221) Antibody
3776   Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb
4904   Stat3 (79D7) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
9131   Phospho-Stat3 (Tyr705) Antibody
9139   Stat3 (124H6) Mouse mAb
9145   Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb
9175   Stat1 (42H3) Rabbit mAb
9351   Phospho-Stat5 (Tyr694) Antibody
9939   Stat Antibody Sampler Kit

Select antibodies in this kit are sold under a license from Chemicon International, Inc. relating to U.S. Patent No. 5,658,791.
Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Jak Isoform Sampler Kit

Immune Cell Signaling Pathways

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