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#9946 Forkhead Signaling Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#9946T1 Kit107,000
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FoxO1/3/4 製品一覧

9946 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody #9464 20 µl WB, IP H, M, R, Mk 78 to 82, 95 Rabbit
Phospho-FoxO1 (Ser256) Antibody #9461 20 µl WB H, M, R, Mk C, Z, B 82 Rabbit
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb #2599 20 µl WB H, M, Mk 65, 78 to 82, 95 Rabbit
FoxO1 (C29H4) Rabbit mAb #2880 20 µl WB, IP, IHC-P, IF-IC, F H, M, R, Mk 78 to 82 Rabbit IgG
Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb #13129 20 µl WB H, M, R, Mk 97 Rabbit IgG
Phospho-FoxO3a (Ser318/321) Antibody #9465 20 µl WB, IP H, M, R, Mk C 97 Rabbit
FoxO4 Antibody #9472 20 µl WB H, M, R, Mk 65 Rabbit
FoxO3a (D19A7) Rabbit mAb #12829 20 µl WB, IP, IHC-P, IF-IC, F H, M, R 82 to 97 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, serum starved or serum treated, using Phospho-Fox01 (Ser256) Antibody.

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells, serum starved or serum treated, using Phospho-Fox01 (Thr24)/Fox03a (Thr32) Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos and Jurkat cells, using Fox04 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved and serum-treated NIH/3T3 cells as well as untreated and LY294002/Wortmannin-treated MDA-MB-468 cells, using Phospho-Fox03a (Ser318/321) Antibody (upper) or Akt Antibody #9272 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from IGROV-1 and COS-7 cells using FoxO1 (C29H4) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells treated with either Calyculin A (#9902) or LY294002 (#9901), NIH3T3 and COS-7 cells using Phospho-FoxO1 (Thr24)/(FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb to detect FoxO1, FoxO3a and FoxO4 when phosphorylated at the Thr24, Thr32, and Thr28 positions, respectively (left panel). Total FoxO1, FoxO3a and FoxO4 were detected using FoxO1 (C29H4) Rabbit mAb (#2880), FoxO3a (75D8) Rabbit mAb (#2497) and FoxO4 Antibody (#9472), respectively (right panel).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T, MRK-nu-1 and Jurkat cells using FoxO3a (D19A7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untreated (-) or treated with LY294002 #9901 (50 μM, overnight; +) and Wortmannin #9951 (1 μM, overnight; +), using Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb (upper) or FoxO3a (D19A7) Rabbit mAb #12829 (lower).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon using FoxO1 (C29H4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells treated with either Calyculin A (#9902) or LY294002 (#9901) using Phospho-FoxO1 (Thr24)/(FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb. The phospho-specificity of the antibody was verified by treating the membrane in the absence (-) or presence (-) of calf intestinal phosphatase (CIP) after western transfer.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FoxO3a (D19A7) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using FoxO1 (C29H4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded PC-3 (upper) and MRK-nu-1 (lower) cell pellets, treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (left) or LY294002 #9901 (right), using FoxO3a (D19A7) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using FoxO1 (C29H4) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using FoxO3a (D19A7) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded IGROV-1 cell pellets, LY294002-treated (left) or insulin-treated (right), using FoxO1 (C29H4) Rabbit mAb. Note the cytoplasmic localization of FoxO1 upon Akt activation.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded metastatic SKOV3 tumor in mouse lung using FoxO3a (D19A7) Rabbit mAb. Note nuclear staining in adjacent normal lung.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded SKOV-3 xenograft using FoxO1 (C29H4) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 cells (green) and MRK-NU-1 cells (blue) using FoxO3a (D19A7) Rabbit mAb. Anti-rabbit (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of PC-3 cells, treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (left) or LY294002 #9901 (right), using FoxO3a (D19A7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of IGROV-1 (green) and Jurkat (blue) using FoxO1 (C29H4) Rabbit mAb. Anti-rabbit (H+L), F(ab')2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of IGROV-1 cells, LY294002-treated (left) or insulin-treated (right), using FoxO1 (C29H4) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

バックグラウンド

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

使用文献
 
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7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Forkhead Signaling Antibody Sampler Kit

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