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#9967 Actin Reorganization Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年3月25日15時35分 現在
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#9967T1 Kit99,000
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Actin 製品一覧

9967 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313 20 µl WB, IF-IC H, M, R, Mk, B 19 Rabbit IgG
Cofilin (D3F9) XP® Rabbit mAb #5175 20 µl WB, IF-IC H, M, R, Mk, Dg 19 Rabbit IgG
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb #3726 20 µl WB, IHC-P, IF-IC, E-P H, M, R, Mk B 80 Rabbit IgG
Ezrin/Radixin/Moesin Antibody #3142 20 µl WB H, M, R, Mk, B X, Dg 75 Moesin. 80 Ezrin and Radixin. Rabbit
Phospho-VASP (Ser157) Antibody #3111 20 µl WB H, M, R, Mk, GP 50 Rabbit
Phospho-VASP (Ser239) Antibody #3114 20 µl WB H, M, R, Mk 48, 50 Rabbit
VASP (9A2) Rabbit mAb #3132 20 µl WB, IP, IF-IC H, M, R, Hm, Mk, B 46, 50 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin), E-P=Peptide ELISA (DELFIA), IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, B=Bovine, Dg=Dog, GP=Guinea Pig, Hm=Hamster

貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of untreated or Staurosporine #9953 treated (1 uM, 3 hrs.) HeLa cells using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb #3726 (upper) and Ezrin/Radixin/Moesin Antibody #3142 (lower).

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and C6 cells, untreated, 8-Br-cGMP-treated, 8-Br-cAMP-treated or forskolin-treated as indicated, using Phospho-VASP (Ser239) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ezrin/Radixin/Moesin Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Cofilin (D3F9) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Forskolin #3828 (+), using Phospho-VASP (Ser157) Antibody (upper) and VASP (9A2) Rabbit mAb #3132 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using VASP (9A2) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of COS cells, untreated or λ phosphatase-treated, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (upper) or Cofilin Antiobdy #3312 (lower).

Western Blotting

Western Blotting

Western blot analysis of various cell extracts, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and C6 cells, untreated, 8-Br-cGMP-treated, 8-Br-cAMP-treated or forskolin-treated as indicated, using Phospho-VASP (Ser157) Antibody.


IP

IP

Immunoprecipitation of VASP from HeLa cell extracts using VASP Rabbit mAb. The same antibody was used for Western detection.

Western Blotting

Western Blotting

Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Cofilin (D3F9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Forskolin #3828 (+), using Phospho-VASP (Ser239) Antibody (upper) and VASP (9A2) Rabbit mAb #3132 (lower).

Western Blotting

Western Blotting

Western blot analysis of Staurosporine #9953 treated (1 μM, 3 hr) or untreated HeLa cells using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb (upper) and Ezrin/Radixin/Moesin Antibody #3142 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells either untreated (left) or λ phosphatase-treated (right), using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HUVE cells using VASP (9A2) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian endometrioid adenocarcinoma using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, untreated (left) or λ phosphatase treated (right), using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left), treated with Staurosporine #9953 (1 μM, 1hr; center), or λ phosphatase (right) labeled with Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).

ELISA-Peptide

ELISA-Peptide

Validation of Phospho-Ezrin (Thr567)/ Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit mAb (ELISA Specific) in peptide DELFIA® assay using phospho- and nonphospho-peptide controls, and DELFIA® secondary antibodies (available from Perkin Elmer Life and Analytical Sciences).


バックグラウンド

Ubiquitous actin protein comprises the major structural component of the eukaryotic cytoskeleton. The formation and continual reorganization of the actin cytoskeleton is a key step in many biological processes, including cell motility, cytokinesis, endocytosis, embryonic development, tissue regeneration and the stress response (1). The small protein cofilin is one of a conserved family of actin-binding proteins that promote actin filament regeneration by severing preexisting filaments (2). Phosphorylation of cofilin at Ser3 by LIMK or TESK inhibits cofilin severing activity (3-5). Ezrin, radixin, and moesin (ERM) proteins function as linker proteins and signal transducers between the plasma membrane and actin cytoskeleton. These proteins are involved in cell adhesion, membrane ruffling, and microvilli formation (6,7). Interactive cytosolic ERM proteins exist as monomers or dimers that form both intra- and intermolecular associations through their amino- and carboxy-terminal domains (8). Phosphorylation at carboxy-terminal threonine residues (Thr567 of ezrin, radixin at Thr564 and Thr558 of moesin) may alter protein conformation and disrupt these protein associations and result in ERM protein activation (9,10). Vasodilator-stimulated phosphoprotein (VASP) is an adaptor protein that links the cytoskeleton with signal transduction pathways to act in fibroblast migration, platelet activation and axon guidance (11,12). Three phosphorylation sites (Ser157, Ser239, and Thr278) have been identified, with phosphorylation of Ser239 by PKG serving as a marker for nitric oxide and cGMP signaling (13). VASP Ser157 can act as a substrate for both PKA and PKC (14,15). Active VASP appears to promote actin polymerization by restricting actin filament capping, with PKA phosphorylation inhibiting this anti-capping activity (16).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
3111   Phospho-VASP (Ser157) Antibody
3114   Phospho-VASP (Ser239) Antibody
3132   VASP (9A2) Rabbit mAb
3142   Ezrin/Radixin/Moesin Antibody
3313   Phospho-Cofilin (Ser3) (77G2) Rabbit mAb
4967   β-Actin Antibody
4968   Pan-Actin Antibody
4970   β-Actin (13E5) Rabbit mAb
5175   Cofilin (D3F9) XP® Rabbit mAb
7074   Anti-rabbit IgG, HRP-linked Antibody

DRAQ5 is a registered trademark of Biostatus Limited.
DELFIA is a registered trademark of PerkinElmer, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Actin Reorganization Antibody Sampler Kit

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