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#9968 Rho-GTPase Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#9968T1 Kit92,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Cdc42 (11A11) Rabbit mAb #2466 20 µl WB H, M, R, B X 21 Rabbit
Phospho-Rac1/cdc42 (Ser71) Antibody #2461 20 µl WB H M, R 28 Rabbit
RhoA (67B9) Rabbit mAb #2117 20 µl WB H, M, R, Mk, B 21 Rabbit IgG
RhoB Antibody #2098 20 µl WB H, M, R, Mk 21 Rabbit
RhoC (D40E4) Rabbit mAb #3430 20 µl WB H, M, Mk R 21 Rabbit IgG
Rac1/2/3 Antibody #2465 20 µl WB, F H, M, R, Mk, B X 21 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, B=Bovine, Mk=Monkey

貯法
-20℃
※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。
社内データ

Western Blotting

Western Blotting

Western blot analysis of A431 cell extracts, untreated (-) or EGF treated (+), using Phospho-Rac1/cdc42 (Ser71) Antibody (upper) or Rac1/Cdc42 Antibody #4651 (lower).

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS, C6, NIH/3T3 and HEK293 cells, using Rac1/2/3 Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lysates, using Cdc42 (11A11) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using RhoA (67B9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain tissue using RhoB Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using RhoC (D40E4) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using Rac1/2/3 Antibody (blue) compared to a nonspecific negative control antibody (red).

Western Blotting

Western Blotting

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoB Antibody.


Western Blotting

Western Blotting

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoA (67B9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoC (D40E4) Rabbit mAb.

バックグラウンド

The Rho family of small GTPases, including Rho, Rac, and cdc42, act as molecular switches to regulate processes such as cell migration, adhesion, proliferation and differentiation (1). RhoA, RhoB and RhoC are all highly homologous but appear to have divergent biological functions. The best characterized of these proteins, RhoA, regulates acomysin contractility, cytokinesis, focal adhesion assembly and cell polarity (2-5). Mammalian Rac exists as three isoforms (Rac1, Rac2 and Rac3) that show high sequence similarity. Well-characterized Rac1 and cdc42 are ubiquitously expressed and play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, and cell growth and development (6). Phosphorylation of Rac1 at a putative Akt site (Ser71) may limit Rac1 activity through inhibition of GTP binding (7). Rac2 is expressed in cells of hematopoietic origin, while Rac3 is highly expressed in brain and in many other tissues. The Vav family of guanine-nucleotide exchange factors mediates activation of Rho/Rac family small GTPases (8). Negative regulation of Rho-activity members of the p190 RhoGAP family (p190-A and p190-B) may be controlled by Src phosphorylation of Tyr residues, activating the p190 GAP domain (8-10). Furthermore, Rho GDP dissociation inhibitor (RhoGDI) associates with Rho/Rac to negatively regulate nucleotide exchange membrane localization (11).

使用例
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関連製品
2117   RhoA (67B9) Rabbit mAb
2461   Phospho-Rac1/cdc42 (Ser71) Antibody
2462   Cdc42 Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

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