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#9970 PDGF Receptor β Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月17日15時10分 現在
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PDGFRb 製品一覧

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-PDGF Receptor β (Tyr740) (32A9) Rabbit mAb #3168 20 µl WB M H 190 Rabbit IgG
Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb #4549 20 µl WB H, M R 190 Rabbit IgG
Phospho-PDGF Receptor β (Tyr771) (76D6) Rabbit mAb #3173 20 µl WB M H 190 Rabbit IgG
Phospho-PDGF Receptor β (Tyr1009) (42F9) Rabbit mAb #3124 20 µl WB, IP H, M 190 Rabbit
Phospho-PDGF Receptor β (Tyr1021) (6F10) Rabbit mAb #2227 20 µl WB H, M, R 190 Rabbit IgG
PDGF Receptor β (28E1) Rabbit mAb #3169 20 µl WB, IP, IHC-P, IHC-F, IF-IC H, M, R 190 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: M=Mouse, H=Human, R=Rat

貯法
-20℃
※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Phospho-PDGF Receptor β (Tyr740) (32A9) Rabbit mAb specifically binds to tyrosine phosphorylated PDGF receptor-β, but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-PDGF Receptor beta (Tyr740) (32A9) Rabbit mAb (upper) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using PDGF Receptor β (28E1) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells untreated or treated with PDGF-BB, using Phospho-PDGF Receptor beta (Tyr771) (76D6) Rabbit mAb (upper) or PDGF Receptor beta (28E1) Rabbit mAb #3169.

Western Blotting

Western Blotting

Western blot analysis of extracts of NIH/3T3 cells untreated or treated with PDGF-BB, using Phospho-PDGF Receptor β (Tyr1021) (6F10) Rabbit mAb (upper), or PDGF Receptor β (2B3) Mouse mAb (#3175) (lower).

Western Blotting

Western Blotting

Western blot analysis of cell extracts from NIH/3T3 cells unstimulated or stimulated with PDGF-BB (100 ng/ml for 5 min), using Phospho-PDGF Receptor-β (Tyr1009) (42F9) Rabbit mAb (upper) or PDGF receptor-β (2B3) Mouse mAb #3175 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts of NIH/3T3 cells, untreated or stimulated with Platelet-Derived Growth Factor (PDGF) #9909, using Phospho-PDGF Receptor-β (Tyr751) (C63G6) Rabbit mAb (upper) or PDGF Receptor-β (2B3) Mouse mAb #3175 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PDGF Receptor β (28E1) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human glioblastoma using PDGF Receptor β (28E1) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded U-87MG cells, showing membrane localization, using PDGF Receptor β (28E1) Rabbit mAb.

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen U-87MG xenograft using PDGF Receptor beta (28E1) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells, serum-starved (left) or PDGF-treated (right), using PDGF Receptor beta (28E1) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


バックグラウンド

Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8).

使用例
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3166   Phospho-PDGF Receptor β (Tyr751) (88H8) Mouse mAb
3170   Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb
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DRAQ5 is a registered trademark of Biostatus Limited.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

PDGF Receptor β Antibody Sampler Kit

Immune Cell Signaling Pathways

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