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#9971 Toll-like Receptor Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#9971T1 Kit75,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Toll-like Receptor 1 Antibody #2209 20 µl WB H Mk 86 Rabbit
Toll-like Receptor 2 (D7G9Z) Rabbit mAb #12276 20 µl WB, IP H 90-105 Rabbit IgG
Toll-like Receptor 7 (D7) Rabbit mAb #5632 20 µl WB, IP H 140 Rabbit IgG
Toll-like Receptor 9 (D2C9) Rabbit mAb #5845 20 µl WB, IP H 130 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human

貯法
-20℃
※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with human TLR1, using Toll-like Receptor 1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Toll-like Receptor 7 (D7) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Toll-like Receptor 9 (D2C9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of THP-1 and MUTZ-3 cells using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from SR cells using Toll-like Receptor 1 Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human TLR7 (+), using Toll-like Receptor 7 (D7) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing HA-tagged full-length human TLR2 (hTLR2-HA; +), using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.

IP

IP

Immunoprecipitation of TLR2 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Toll-like Receptor 2 (D7G9Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Toll-like Receptor 2 (D7G9Z) Rabbit mAb.


バックグラウンド

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.

使用例
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Tween is a registered trademark of ICI Americas, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Toll-like Receptor Antibody Sampler Kit

Immune Cell Signaling Pathways

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