免疫沈降して得られたDNAのインプットクロマチンに対する値をグラフにしました。#9005 SimpleChIP® Plus Chromatin IP Kit (Magnetic Beads) が、競合製品に比べてシグナルの増幅が顕著かつ高感度であることが実証されました。
Figure B. Competitor's Sonication-based Tissue ChIP Kit
SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic beads) #9005 (A) compared to a competitor’s sonication-based Tissue Chromatin IP kit (B). Mouse liver was cross-linked and disaggregated into a single-cell suspension using a tissue disaggregator. The chromatin was prepared and fragmented according to the manufacturer's recommendations and chromatin immunoprecipitations were performed using the indicated ChIP-validated antibodies. Purified DNA was analyzed by quantitative real-time PCR using SimpleChIP® Mouse GAPDH Intron 2 Primers #8986, SimpleChIP® Mouse AFM Intron 2 Primers #7269, SimpleChIP® Mouse HoxA1 Promoter Primers #7341, and SimpleChIP® Mouse HoxD10 Exon 1 Primers #7429. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to 1).