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SimpleChIP® Plus Enzymatic Chromatin IP Kits

クロマチン免疫沈降(ChIP)特集パンフレットPDF  2.2 MB

CSTの新しいSimpleChIP® Plus Chromatin IPキットは、培養細胞や組織サンプルの内在性レベルのタンパク質-DNA相互作用を検出するのに最適です。CSTはクロマチンの断片化に酵素消化を採用しており、キットには酵素消化-クロマチン免疫沈降 (ChIP) 実験を、組織サンプルで迅速かつ簡便に行うために必要な全ての試薬が含まれています (ChIPアッセイ30回分)。また、ポジティブとネガティブコントロールも含まれ、プロテインGアガロースビーズ (#9004) およびプロテインG磁気ビーズ (#9005) のキットをご用意しています。

SimpleChIP® Plus Chromatin IP Kit

SimpleChIP® Plus Enzymatic Chromatin IPキットの利点

Mouse Kidney ChIP (Protein G Magnetic Beads)

Figure A.

Figure A, Mouse Kidney Magnetic Beads

Figure B.

Figure B, Mouse Kidney Magnetic Beads

Mouse Spleen ChIP (Protein G Magnetic Beads)

Figure C.

Figure C, Mouse Spleen Magnetic Beads

Figure D.

Figure D, Mouse Spleen Magnetic Beads

Figure Caption: Mouse kidney and spleen were prepared and disaggregated into a single-cell suspension using a tissue disaggregator, as described in the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The cells were then cross-linked with formaldehyde, and chromatin was prepared and digested. Chromatin immunoprecipitations were performed using the chromatin from mouse kidney (panels A and B) and spleen (panels C and D), and the indicated ChIP-validated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Mouse GAPDH Intron 2 Primers #8986, Mouse RPL30 Intron 2 Primers #7015, SimpleChIP® Mouse HoxA1 Promoter Primers #7341, and SimpleChIP® Mouse HoxD10 Intron 1 Primers #7429. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to 1).

SimpleChIP® Plus Enzymatic Chromatin IP Kits

No. Name Applications Reactivity
9004 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) ChIP H, M, R, Mk
9005 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) ChIP H, M, R, Mk

Applications Key: ChIP=Chromatin Immunoprecipitation 
Reactivity Key: H=Human  M=Mouse  R=Rat  Mk=Monkey 

SimpleChIP® Plus Enzymatic Chromatin IP Kits

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