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Motif Antibodies (モチーフ抗体)

Introduction

Motif specific antibodies are proprietary to CST (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents) and are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific kinase substrate, binding motif, or other phosphorylation motifs. These phospho-motif antibodies are useful to identify and quantify substrates and biomarkers for kinases from many branches of the human kinome.

Motif antibodies can be employed in a variety of platforms to detect specific phosphorylated protein sequences and can be used in combination with separation analysis such as 2D gel SDS PAGE to characterize or identify specific phosphoproteins.

These antibodies can also be applied in conjunction with PhosphoScan®, CST’s proprietary masspectrometry technology for post-translational modification site discovery and quantification.

Motif antibodies can also be used for drug discovery as detection antibodies for in vitro enzymatic assays including kinase and phosphatase assays.

Product Categories

PhosphoScan Motif Antibodies

Small Human Kinome Small Human Kinome Key

View the applications key.

Phospho-Serine

No. Antibody Name Applications Motif Recognized
9615 Phospho-(Ser) Kinase Substrate Antibody Sampler Kit
9606 Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb W, IP, E-P (K/R)XXS*P
9601 Phospho-(Ser) 14-3-3 Binding Motif Antibody W, IP, IHC-P, E-P (K/R)XXS*P
9607 Phospho-ATM/ATR Substrate Motif [pSQ] MultiMAb™ Rabbit mAb mix W, IP S*Q
6967 Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab™ Rabbit mAb mix W, IP, E-P (K/R)XS*X(K/R)
2325 Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb W, IP, IHC-P, E-P PXS*P, S*PX(K/R)
5501 Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) IP PXS*P, S*PX(R/K)
9477 Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix W, IP, E-P (K/H)S*P
2261 Phospho-(Ser) PKC Substrate Antibody W, IP, E-P (K/R)XS*(Hyd)(K/R)
2981 Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody W, IP, E-P RX(F/Y)XS*

Phospho-Threonine

No. Antibody Name Applications Motif Recognized
14371 Phospho-CDK Substrate [pTPXK] (D9V5N) Rabbit mAb W, IP
9391 Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) W, IHC-P, E-P T*P
5243 Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb W, IP, E-P ST*P
2351 Phospho-Threonine-X-Arginine Antibody W, IHC-P, E-P T*X(K/R)
3004 Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb W, IP, E-P T*PE, T*P
9386 Phospho-Threonine (42H4) Mouse mAb W, IP, E-P T*
9381 Phospho-Threonine Antibody (P-Thr-Polyclonal) W, IP, E-P T*
8859 Phospho-Threonine Antibody (P-Thr-Polyclonal) (Magnetic Bead Conjugate) IP T*
8781 Phospho-Threonine Antibody (P-Thr-Polyclonal) (Sepharose Bead Conjugate) IP T*
6949 Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate) W, E-P T*

Phospho-Serine / Threonine

No. Antibody Name Applications Motif Recognized
9920 Phospho-(Ser/Thr) Kinase Substrate Antibody Sampler Kit
9614 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb W, IP, IHC-P, E-P RXX(S*/T*)
6950 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate) W RXX(S*/T*)
8050 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Magnetic Bead Conjugate) IP RXX(S*/T*)
9646 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate) IP RXX(S*/T*)
9611 Phospho-(Ser/Thr) Akt Substrate Antibody W, IP, IHC-P, E-P (K/R)XX(S*/T*)
10001 Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb W, E-P RXRXX(S*/T*)
5759 Phospho-AMPK Substrate Motif [LXRXX(pS/pT)] MultiMab™ Rabbit mAb mix W, IP, E-P (L/M)XRXX(S*/T*), RXX(S*/T*)
2909 Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb W L(S*/T*)Q
2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody W, IP, IHC-P, E-P L(S*/T*)Q
6966 Phospho-ATM/ATR Substrate Motif [(pS/pT)QG] MultiMab™ Rabbit mAb mix W, IP (S*/T*)QG, (S*/T*)Q
8738 Phospho-CK2 Substrate Motif [(pS/pT)DXE] MultiMab™ Rabbit mAb mix W (S*/T*)DXE
9634 Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb W, IP, E-P (F/K)XX(F/Y)(S*/T*)(F/Y)
9631 Phospho-(Ser/Thr) Phe Antibody W, IP, E-P (F/Y/W)(S*/T*)(F)
9624 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb W, IP, E-P (K/R)(K/R)X(S*/T*)
9621 Phospho-(Ser/Thr) PKA Substrate Antibody W, IP, IHC-P, E-P (K/R)(K/R)X(S*/T*)
4381 Phospho-(Ser/Thr) PKD Substrate Antibody W, E-P LXRXX(S*/T*)

Phospho-Tyrosine

No. Antibody Name Applications Motif Recognized
8954 Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix W, IP, IF-IC, F Y*
14219 Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix (Biotinylated) W Y*
12695 Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix (HRP Conjugate) W Y*
14017 Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix (Magnetic Bead Conjugate) WIP Y*
14005 Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix (Sepharose Bead Conjugate) IP Y*
9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100) W, IP, IHC-P, IF-P, IF-F, IF-IC, F, E-P Y*
9414 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate) F Y*
9415 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 647 Conjugate) IF-IC, F Y*
9417 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) W, IP, IHC-P Y*
5465 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) W, E-P Y*
8095 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate) IP Y*
9419 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate) IP Y*
9416 Phospho-Tyrosine Mouse mAb (P-Tyr-102) W, IP, IHC-P, F, E-P Y*

Nitro-Tyrosine

No. Antibody Name Applications Motif Recognized
9691 Nitro-Tyrosine Antibody W Y-NO2

Methyl-Lysine

No. Antibody Name Applications Motif Recognized
14117 Di-Methyl Lysine Motif [dme-K] MultiMab™ Rabbit mAb mix W, IP Me-K
14680 Tri-Methyl Lysine Motif [tme-K] (D1L1X) Rabbit mAb W Me-K

Acetylated-Lysine

No. Antibody Name Applications Motif Recognized
9814 Acetylated-Lysine (Ac-K-100) MultiMab™ Rabbit mAb mix W, IP, ChIP, E-P Ac-K
6952 Acetylated-Lysine (Ac-K-100) MultiMab™ Rabbit mAb mix (HRP Conjugate) W Ac-K
9681 Acetylated-Lysine Mouse mAb (Ac-K-103) W, E-P Ac-K
9441 Acetylated-Lysine Antibody W, IP, IHC-P, IF-IC, ChIP, E-P Ac-K

Methyl-Arginine

No. Antibody Name Applications Motif Recognized
8015 Mono-Methyl-Arginine [mme-R] MultiMab™ Rabbit mAb mix W, IP, E-P Me-R
8711 Mono-Methyl Arginine (R*GG) (D5A12) Rabbit mAb W, IP, E-P Me-RGG
13522 Asymmetric Di-Methyl Arginine Motif [adme-R] MultiMab™ Rabbit mAb mix W Me-R
13222 Symmetric Di-Methyl Arginine Motif [sdme-RG] MultiMab™ Rabbit mAb mix W Me-RG

Cleaved-Aspartic acid

No. Antibody Name Applications Motif Recognized
8698 Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab™ Rabbit mAb mix W DE(T/S/A)D

Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services at ptmscan@cellsignal.com.


Applications Key

ChIP
Chromatin Immunoprecipitation
E-P
ELISA Peptide
ELISA
Enzyme Linked Immunosorbent Assay
F
Flow cytometry
IC
Immunocytochemistry
IC-ABC
Immunocytochemistry (Avidin Biotin Complex)
ICW
In-Cell Western
IF-F
Immunofluorescence (Frozen)
IF-IC
Immunofluorescence (Immunocytochemistry)
IF-P
Immunofluorescence (Paraffin)
IHC-F
Immunohistochemistry (Frozen)
IHC-P
Immunohistochemistry (Paraffin)
IP
Immunoprecipitation
P-Scan
PhosphoScan
W
Western blot

アプリケーション

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